Effects of Butterfly Pea Extracts on Phagocytic Activity of Blood Polymorphonuclear Leukocytes and Muscular Lipid Peroxidation in Rabbits.

Sixteen 35-day-old male crossbred rabbits (New Zealand white × Thai native breed) with an initial weight of 484 ± 11.3 g were randomly divided into two groups of eight, constituting control and treatment groups. The treatment group was orally administered a crude extract of butterfly pea (Clitoria ternatea L.) at 0.5 g/kg body weight from weaning (at 35 days) to slaughter (at 90 days). The effects on the phagocytic activity of blood polymorphonuclear leukocytes, serum biochemistry, meat quality, muscular lipid peroxidation, the apparent digestibility of dry matter and nutrients, and gut histology were studied. The results revealed that the phagocytic function of circulating leukocytes (75 and 90 days) and alveolar macrophages (90 days) did not differ between the two groups. At slaughter, treated rabbits had lower blood urea nitrogen concentrations and higher liver weight than control rabbits (p < 0.05). After chilling at 4 °C for 24 h, a lower meat pH and the alteration of meat color (brighter, less yellow, lower hue angle, and decreased color saturation) were observed in the treated group (p < 0.05). Furthermore, lipid peroxidation (measured at 3, 5, and 7 storage days) in the meat of treated rabbits was lower than in controls (p < 0.05). The apparent digestibility of organic matter and ether extract (analyzed at 46 days for 4 days) was improved in the treated group (p < 0.05), whereas gut histology was unaffected. In conclusion, butterfly pea extract supplementation did not affect phagocytic function but led to a modification in meat color, delayed lipid peroxidation, and improved digestibility.

Detection of Booroola Polymorphism of Bone Morphogenetic Protein Receptor 1b and Embrapa Polymorphism of Growth Differentiation Factor 9 in Sheep in Thailand.

This study aimed to investigate the appearance and frequencies of the Booroola polymorphism of the bone morphogenetic protein receptor 1b (BMPR1B) gene (FecB) and the Embrapa polymorphism of the growth differentiation factor 9 (GDF9) gene (FecGE) in sheep in Thailand. A total of 454 crossbred sheep blood samples were collected from four provinces in Thailand during August 2022 to July 2023. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used to identify the FecB and FecGE genotypes. The history of ewe birth types was collected from the owners to analyze the association between fecundity (Fec) genotypes and the history of birth types. The genotypic frequencies of FecB for homozygous genotype (B/B), heterozygous genotype (+/B), and wildtype (+/+) were 0.22%, 1.54%, and 98.24%, respectively. Meanwhile, the genotypic frequencies of FecGE for homozygous genotype (E/E), heterozygous genotype (+/E), and wildtype (+/+) were 0.00%, 2.42%, and 97.58%, respectively. Furthermore, three ewes exhibited both FecB and FecGE genotypes. Fisher's exact test revealed that possession of the FecB genotype was associated with multiple births (p < 0.01). Both FecB and FecGE mutations were identified in crossbred sheep in Thailand. Sheep containing FecB allele could be alternative candidates to be selected to improve the prolificacy of crossbred sheep in Thailand.

Development of in-house ELISA based on recombinant gag proteins of small ruminant lentiviruses isolated from goats in Thailand.

Small ruminant lentiviruses (SRLVs), are grouped in Retroviridae family, remain a significant loss in the small ruminant husbandry. As a result of unavailability of vaccine and effective treatment, the diagnosis plays a crucial role for the control of SRLV infection. However, the major challenge of diagnosis of SRLV infection is the genetic and antigenic variability of the viruses that can lead to a failure in serological detection. This study investigated the circulating strains of the viruses in goats in Thailand and an in-house ELISA was developed. The coding sequences for gag protein were optimized, synthesized, and expressed in Escherichia coli for increasing the sensitivity of ELISA test. A total of 365 serum samples were examined against the recombinant protein in an in-house ELISA. The results showed that the recombinant gag achieves 96.67% sensitivity and 93.18% specificity as compared with the commercially available ELISA test kit.

Effect of claw blocks on the healing duration and lesion severities of claw lesions in lame cows in Western Thailand.

Background and Aim: Lameness is a major complication in dairy cattle affecting health and milk production. Several factors are found to contribute to this condition and specific treatments are required, including the process of claw trimming. The elevation of the claw, such as with the application of a claw block, was reported to be beneficial in the more severe cases. This study aimed to determine the efficiency of a claw block on claw lesions of lame cows in dairy farms in Western Thailand. Materials and Methods: Locomotion scores of 376 dairy cows were determined by a veterinarian using a scale of 1-5 (1 = normal; 5 = severely lame) at the time of the visit. Cows with a score of 3 or greater were defined as clinically lame. In total, 134 clinically lame cows from 11 dairy farms in the Kanchanaburi and Ratchaburi provinces were included in the analysis. Claw lesions included a white line abscess, bruised sole, sole ulcer, sole abscess, white line separate, and double soles. Wooden or rubber claw blocks were applied to the unaffected claw of the same hoof as the injured claw of 116 cows, which were classified as the treatment cases, and 18 cows were left untreated and classified as the control cases. Each cow was checked on every week of the healing process for 2 months unless the cow was culled earlier. Survival analysis was based on the Kaplan-Meier estimator and Cox Proportional Hazard regression. Results: The median healing time for lame cows with and without claw blocks was 21 and 36 days, respectively. After adjusting for the lesion severity and type, the lame cows with and without a claw block had hazard ratios of 2.16 and 3.08, respectively. The healing times between the four lesion types in cows with a claw block were not significantly different. The healing time was longer in lame cows, with a severity score of 4. Conclusion: The results from this study reveal that the treatment of lame cows with claw blocks promoted the healing capacity of claw lesions after claw trimming.

Economic and value chain analysis to support an investigation and risk mitigation efforts on Marek's disease in layers in the southern part of Thailand.

Background and Aim: Marek's disease (MD) is a common lymphoproliferative disease affecting chickens and causing economic losses in commercial poultry. The MD outbreak was noticed in the southern part of Thailand in 2019. The suspected cases were found with an abnormal number of cases of layers dying with clinical signs, for example, weakness and emaciation, with evidence of MD gross lesions. This study aimed to raise awareness of the MD outbreak through value chain analysis (VCA), identifying associated possible risk factors, and estimating the associated economic impact. Materials and Methods: Value chain analysis, including seasonal calendar, value chain diagram, and layer movement mapping of the layer industry, was conducted. High-risk stakeholders were identified on the basis of risk practices and interactions between stakeholders. A case-control study was conducted to determine risk factors associated with the MD outbreak on layer farms, and partial budget analysis was used to estimate economic losses associated with MD. Results: The value chain diagram showed the linkages between stakeholders, including estimation of the percentage of products moved from one stakeholder group to another and the negotiated price. Fourteen out of 35 layer farms were case farms. Farm size and source of birds were significantly associated with the MD outbreak. The MD outbreak caused total economic losses of 295,823 USD. Farms that slaughtered infected birds with additional revenues incurred losses of 140,930 USD, whereas farms that culled infected birds without additional revenue returned incurred losses of 1995 USD. Conclusion: The VCA provided a better understanding of the layer and egg businesses in South Thailand and guided the development of questionnaires for outbreak investigation. The potential risk factor findings suggested the need for further exploration of the source of the MD outbreak.

Factors affecting first ovulation in postpartum dairy cows under tropical conditions: A review.

It is documented that the reproductive performance of postpartum dairy cows is influenced by both internal and external factors. One of the most important reproductive performance indices is the first ovulation postpartum. This review aimed to describe factors affecting first ovulation postpartum in dairy cows, particularly those raised under tropical climates. Negative energy balance and its consequences; metabolic disorders; uterine pathology; udder health; lameness; and heat stress are discussed for their potential effects on the first ovulation postpartum. Understanding the underlying mechanisms of those affecting factors would assist in justifying the treatment and prevention plans in order to optimize the reproductive performance of postpartum dairy cows.

An economic analysis of high milk somatic cell counts in dairy cattle in Chiang Mai, Thailand.

Mastitis in dairy cattle can lead to significant financial losses due to a reduction in milk yield, the withdrawal period after treatment when milk cannot be sold, and an increase in somatic cell count (SCC) which can reduce the milk's per liter commercial value. Dairy cooperatives point at high-SCC problems as an important factor leading to suboptimal levels of milk quantity and quality. This study aims at describing farm characteristics and milking practices associated with high SCC, identifying risk factors, and assessing the economic loss due to high SCC in three dairy cooperatives in Chiang Mai, Thailand. A cross-sectional study was conducted on 208 dairy cattle farms from July to September 2018. Structured interviews were conducted to collect the data. Univariate and multivariate analyses were performed to determine the degree of association between factors and high SCC. A retrospective cost assessment of high SCC was conducted to estimate the losses in affected farms, and two potential coping strategies were assessed: (1) culling and (2) treating the cow. More than 12% of farms had high SCC (SCC > 500,000 cells/ml). Inappropriate vacuum pressure and inappropriate pulsation rate of milking machines were identified as significant risk factors according to the multiple logistic regression (P < 0.01). Both factors can decrease the natural protection of teat tissue, increasing the likelihood of bacterial infection. The average economic loss of high SCC in affected farms was 557 USD for a three-month period. When comparing response strategies (i.e., treatment vs. culling), treating the affected cow was found to be more cost-effective. With a probability of successful treatment of 54%, treating an affected cow leads to 1,158.7 USD in gains over 3 years (vs. doing nothing). The results of this economic analysis can be used to advocate to cooperatives the value of veterinarians and for investigating and treating cases of mastitis.

Effects of body condition at far-off dry period on blood biochemistry, liver triacylglycerol and muscular monocarboxylate transporter-1 mRNA expression in tropical Holstein dairy cows during peripartum period.

Effects of body condition score (BCS) at far-off dry period on blood biochemistry, liver triacylglycerol, and muscular monocarboxylate transporter-1 mRNA expression in tropical Holstein dairy cows during peripartum period were studied. Eight dry cows were divided into two groups of four cows based on their BCS at 8 weeks prepartum: high BCS (HBS) and low BCS (LBS). Blood samples were collected weekly from 8 weeks before expected calving date until 8 weeks postpartum for determination of serum glucose, non-esterified fatty acid (NEFA), and ß-hydroxybutyrate (BHBA). Muscle and liver samples were biopsied at 2 weeks before expected calving date and at 2 weeks postpartum for determination of muscular monocarboxylate transporter-1 (MCT-1) mRNA expression and liver triacylglycerol concentrations. Decreased BCS and elevated serum NEFA and BHBA indicated that LBS cows entered to the NEB period earlier than did HBS cows. LBS cows showed lower liver TAG and higher muscular MCT-1 mRNA expression than did HBS cows. The MCT-1 mRNA expression at 2 weeks prepartum was negatively correlated with BCSs at 2-3 weeks prepartum and at 1-2 weeks postpartum. In conclusion, LBS cows most likely adapted to NEB by upregulation of muscular MCT-1 expression and had lower NEB consequences during postpartum period.

Factors associated with negative energy balance in periparturient dairy cows raised under tropical climate of Thailand-A mini-review.

This review attempted to explain factors associated with negative energy balance (NEB) occurring during the periparturient period in dairy cows raised under tropical climatic conditions. The NEB has long been proven as an inevitable event in periparturient dairy cows. This condition had negative effects on the overall performances of dairy cows, including milk production, reproduction, and health condition. Therefore, periparturient management to overcome the NEB problem is vital for optimizing profit in dairy farming. In most tropical countries such as Thailand, dairy cows have been predominantly kept by small-holder farmers. Consequently, baseline milk yields, feed availability, feeding management, and general farming practices are different from typical commercial dairy farming. Heat stress also plays a crucial role in NEB conditions, and elevated temperature-humidity indexes above-normal conditions are recorded throughout the year. These factors influence the NEB in tropical dairy cows, which could result in different outcomes and consequences. Understanding the affecting components of NEB in dairy cows would help alleviate the severity of the NEB and its consequences, optimizing the dairy cow's performance.

Knowledge, attitude, and practice about rabies prevention and control: A community survey in Nepal.

BACKGROUND AND AIM: Rabies is a fatal zoonosis caused by RNA virus belonging to genus Lyssavirus. Nepal is one of the endemic countries in South Asia for rabies. This study was conducted to better understand the knowledge, attitude, and practice (KAP) of Nepalese community toward rabies across five developmental zones of the country. MATERIALS AND METHODS: The cross-sectional study was carried out by face-to-face interview using structured questionnaires among 5000 respondents of five cities representing each of the five developmental regions of Nepal by adopting random cluster sampling procedure. The respondents were classified into four categories, including gender, age, education, and social status. The responses for KAP variables were analyzed using descriptive and Chi-square test. RESULTS: The male and younger age respondents with higher education and social status were found more knowledgeable than their counterparts in terms of knowledge variables, including cause of rabies, mode of transmission, clinical signs, treatment, and preventive measures of this fatal disease. Similar findings were observed for attitude and practice variables such as vaccination practice, dog sterilization, health-seeking behavior, first aid practice, and use of first aid materials after dog bites. Some respondents in elderly age group still preferred to use traditional and local methods, which were application of turmeric powder and shrubs to cure dog bites rather than seeking medical facilities. CONCLUSION: There is a strong need for rabies awareness programs in the community targeting females, school, and college-level students, older age groups, and economically marginalized communities. The awareness materials need to focus on particular topics such as the risk of rabies, modes of transmission, the importance of first aid, health-seeking behavior following dog bite injuries, and practice preventive measures for their pets and community dogs.

Risk factors associated with acute hepatopancreatic necrosis disease at shrimp farm level in Bac Lieu Province, Vietnam.

BACKGROUND AND AIM: Acute hepatopancreatic necrosis disease (AHPND) is a severe disease in shrimp farms and adversely affected the shrimp industry of Vietnam. So far, the study on risk factors associated with AHPND outbreaks is limited. The objective of this study was to determine the potential risk factors of AHPND at the shrimp farm level in Bac Lieu Province, Vietnam. MATERIALS AND METHODS: Real-time-Polymerase chain reaction was used to analyze data collected from an active surveillance program of shrimp farms in 2017 in the Vinh Tien and Vinh Lac villages, Vinh Thinh commune, Hoa Binh district in Bac Lieu Province, Vietnam. The matched case-control study selected 20 cases and 20 control farms from 134 shrimp farms. In 2018, face-to-face interviews using structured questionnaires were conducted with the farmers of these selected farms. RESULTS: Of the 59 studied variables, seven had p≤0.2 based on bivariate analyses. The results of multivariable analysis showed that the presence of fish-eating birds on shrimp farms was a significant association with AHPND (odds ratio=8, p=0.049). CONCLUSION: To reduce the effect of AHPND, farmers should apply effective methods to manage wild animals such as using a grid or net to cover the pond, combined with improved biosecurity.

Patterns of blood biochemical parameters of peripartum dairy cows raised in either smallholder or semi-commercial dairy farms in Thailand.

BACKGROUND AND AIM: Data about dynamics of serum biochemical parameters and negative energy balance (NEB) related conditions differ among geographical areas or farm types. It should be cautious about applying those data to justify control and prevention strategies of NEB problems. Therefore, dynamics of blood biochemical parameters related to NEB condition of peripartum dairy cows raised in either smallholder (SH) or semi-commercial (SC) farms were studied. MATERIALS AND METHODS: Thirty-two healthy pregnant dry cows were selected from five dairy farms in Western part of Thailand, including 15 and 17 cows from three SH and two SC farms, respectively. Blood samples were collected at 2 weeks before the expected calving date, and 1, 2, 4, and 8 weeks postpartum to determine concentrations of glucose, non-esterified fatty acid (NEFA), and b-hydroxybutyrate (BHBA). Body condition scores (BCSs) and milk yields were also recorded. RESULTS: Dairy cows in both farm types suffered from NEB by elevation of serum NEFA and BHBA, and loss of BCS postpartum. Degrees of elevation in serum NEFA and BHBA were different between farm types. The SC cows showed more BCS loss postpartum, but lower serum NEFA. In the SH cows, even with less BCS loss, the cows showed high serum NEFA concentrations. CONCLUSION: Dairy cows in our study entered NEB condition even with low milk yields. Moreover, elevation of serum NEFA and BHBA postpartum was higher in our studied cows as compared with other studies in high producing cows of commercial dairy farms in temperate areas.

Evolution of antigenic and genetic characteristics of foot-and-mouth disease virus serotype A circulating in Thailand, 2007-2019.

Foot-and-mouth disease (FMD) is a persistent, major economic concern for livestock productivity, which is highly exacerbated by outbreaks in Thailand. FMD virus (FMDV) serotype A is more highly antigenic and genetically diverse than other serotypes, which has important implications for vaccine development as well as selection. Therefore, it is essential to continuously monitor antigenic and genetic changes of field isolates of FMDV serotype A. Here we used antisera against three vaccine strains (A/118/87, A/Sakolnakorn/97, and A/Lopburi/2012) to analyze the antigenicity of 133 field isolates of FMDV serotypes A in Thailand from 2007 to 2019. The majority of the isolates from 2007 to 2008 reacted only with the antiserum against strain A/118/87. In contrast, antigenic analysis revealed broad cross-reactivity and antigenic variations of the isolates from 2009 through 2019 against strains A/Sakolnakorn/97 and A/Lopburi/2012. These results indicate periodic changes in the antigenicity of field isolates of FMDV serotype A. Phylogenetic analysis of the VP1 region revealed that all isolates were of the Sea-97 lineage within the ASIA topotype. Analysis of the L-fragment genome sequences of 30 FMDV isolates collected throughout Thailand revealed highly variable amino acid sequences of VP1 and 3A, with the lowest average identity (94.56 %) and invariant (78.43 %) rates, respectively. The present findings indicate the importance of an active routine surveillance system incorporating antigenic and genetic analysis designated to continually update information about field isolates of FMDV serotype A. Such a system is essential for establishing and improving measures to control FMDV infections in Thailand and in neighboring Asian countries.

Application of discrete choice experiment to assess farmers' willingness to report swine diseases in the Red River Delta region, Vietnam.

A discrete choice experiment (DCE) is carried out to value socio-economic factors influencing the farmer's decision to report swine diseases and to assess the willingness of farmers to report swine diseases. Data were collected between March and July 2015 in two provinces in the Red River Delta, Northern Vietnam, from 196 pig producers by face-to face interview. A conditional logit model is used to measure the relative importance of the socio-economic factors and calculate the expected probability of disease reporting under changes of levels of these factors. Results of the study indicated that the likelihood of compensation and the type of culling implemented (all or only unrecovered pigs) are the two most important factors influencing farmer reporting. Compensation level, movement restriction and delay in compensation payment also have significant impacts on farmer's decision to report animal disease but they are not as important as the above factors. Three different scenarios including changes in six different factors (attributes) are tested to predict probability of animal disease reporting. Under the current situation (uncertainty of being compensated), only 4% of the farmers would report swine disease outbreak to the official surveillance system if the culling policy involves all pigs in affected farms. This number is increased to 26% if culling in affected farms is restricted to unrecovered pigs only. Ensuring certainty of compensation increases reporting probability by up to 50% and 90% if all or only unrecovered pigs are destroyed, respectively. The results of this study are important for improving the performance and sustainability of swine disease surveillance system in Vietnam.

Response of saliva Na/K ratio to changing Na supply of lactating cows under tropical conditions.

BACKGROUND: Factorial determination of the sodium (Na) requirement of heat-stressed lactating cows is hindered by accurate estimates of the Na losses through sweat. Direct studies, therefore, may be needed requiring information on the time course of healthy animals to become Na depleted and the subsequent rate of repletion. The rate of Na depletion and subsequent rate of Na repletion with two levels of dietary Na to lactating dairy cows housed under tropical conditions were investigated using the salivary Na/K. RESULTS: The 12 lactating cows (salivary Na/K ratio 14.6) rapidly developed clinical signs of Na deficiency, including pica, polyuria and polydipsia, reduced body weight and reduced milk yield when fed a low-Na ration (0.33 g kg-1 dry matter (DM)) for 3 weeks. Deficiency symptoms were associated with a rapid decrease in salivary Na/K ratio to <4.3 from 7 to 21 days. Subsequent repletion of the cows with NaCl to a ration concentration of 1.1 or 1.6 g Na kg-1 DM for 5 weeks did not restore salivary Na/K ratio to values of >6. CONCLUSION: A daily Na intake of heat-stressed lactating cows to a ration intake of 1.6 g Na kg-1 DM was insufficient to restore Na deficiency. One week was sufficient to deplete heat-stressed lactating cows of Na, allowing for rapid dose-response studies utilizing the salivary Na/K ratio as a parameter for Na status of cows under tropical conditions. © 2016 Society of Chemical Industry.

Influence of colostral quality on serum proteins in dairy calves raised in smallholder farms in Thailand.

The objective of this study was to analyze the influence of colostral quality on serum proteins in calves. Samples were collected from visited farms in Kasetsart University Veterinary Teaching Hospital at Kamphaeng Saen and Nong Pho Animal Hospital. In total, 35 dairy farms contributed 80 dams and calves' samples. Colostrum samples from 80 dairy cows and blood samples from their calves were taken to evaluate colostral immunoglobulins (Ig) and immunoglobulin G (IgG), and calf serum protein and IgG. Total colostral Ig, colostral and serum IgG, and serum protein were measured by a colostrometer, single radial immunodiffusion, and refractrometer, respectively. Immunoglobulin G and serum protein concentrations increased in the 1st day after birth, and maximum concentrations were seen in the 2nd day and then decreased in the 7th and 14th days. Average ± SD total colostral IgG concentrations at calving date and at 1 and 2 days after calving were 93.85 ± 33.89, 37.11 ± 23.51, and 17.23 ± 9.4 mg/mL, respectively. The profile of total Ig and IgG concentrations in colostrum had a similar pattern, with the maximum concentrations obtained in calving date and rapidly decreased thereafter. Low IgG concentrations were seen in the 7th and 14th day after calving. The calves that were fed with high quality colostrum had higher serum protein at 1 day of age, 7.49 ± 1.01 g/dL, than calves fed with low quality colostrum, 6.40 ± 0.86 g/dL (P < 0.01). The increase in serum protein after first colostrum feeding of high and low quality colostrum was 1.55 ± 1.07 and 0.81 ± 0.69 g/dL, respectively (P = 0.02).

Social network analysis used to assess the relationship between the spread of avian influenza and movement patterns of backyard chickens in Ratchaburi, Thailand.

In this study, we describe the movement and trading patterns of the backyard chicken in Ratchaburi, Thailand by using social network analysis with egocentric approach. From questionnaire results, we found that there is a close relationship between chicken owners' houses and fresh markets, and we concluded that this relationship needs attention from authorities to prevent future outbreaks of avian flu. Control measures should be applied over pathways to prevent and control highly pathogenic avian influenza subtype H5N1 in the backyard farming system. Results of our study may be useful to relevant authorities and researchers seeking to understand how H5N1 spreads in Ratchaburi. This may reflect on the spread of H5N1 throughout Thailand.

An attempt to define the sodium requirement of lactating dairy cows in a tropical environment.

BACKGROUND: Lactating dairy cattle in the tropics may require more sodium (Na) owing to the hot and humid climatic conditions. It is unknown whether the current recommendations on Na for lactating cows can be quantitatively used in tropical countries. This study attempted to define the Na requirement of lactating dairy cows under tropical conditions by measuring Na levels in saliva, milk and faeces. RESULTS: The concentrations of Na and potassium (K) in milk, faeces and serum were not affected by dietary treatments. The amount of Na absorbed by cows fed the basal (low-Na) diet containing 0.4 g Na kg(-1) dry matter (DM) was equal to the amount of Na lost in the milk, showing that these animals were fed an Na-deficient ration. This observation was corroborated by salivary Na and K levels, with the cows on the low-Na diet having salivary Na concentrations below 120 mmol L(-1) in combination with salivary K concentrations above 20 mmol L(-1) (P < 0.05). CONCLUSION: Consumption of a daily ration formulated to contain the current Na requirement set by the NRC appears to provide too much Na for lactating cows under tropical conditions. A tentative value of 1.2 g kg(-1) DM is proposed as the Na requirement for dairy cows under tropical conditions.

Seroprevalence of Toxoplasma gondii in captive felids in Thailand.

The prevalence of antibodies against Toxoplasma gondii was investigated by commercial latex agglutination test kit (Toxocheck-MT 'Eiken') in captive felids maintained at zoos and a wildlife breeding center in different geographic regions of Thailand. Sera from a total of 136 captive felids of 12 species was obtained between 2002 and 2004. The overall seroprevalence of T. gondii was found in 21 of 136 (15.4%) felids. The titers varied from 1:64 (eight samples) to 1:8192 (one sample). The seroprevalence in different geographic regions were from 0% in the northern area to 23% in the southern area. This study suggested a widespread exposure of captive felids to T. gondii in Thailand and this is the first report of serologic analysis for T. gondii in captive felids in Southeast Asia.